Talk:TA cloning

I didn't realize how new this article was; I should have let you work on it more before I made changes. Sorry! Pdcook (talk) 05:26, 17 October 2009 (UTC)[reply]

Don't worry about it at all, I am happy you are editing it! Thanks! --Vishnu2011 (talk) 06:21, 17 October 2009 (UTC)[reply]

Suggest changing the sentence: "It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine overhang[3]" based on the following paper, the sequence should be A rich with a G second to last nucleotide to maximize efficiency? please check this.

Adenosine added on the primer 50 end improved TA cloning efficiency of polymerase chain reaction products, Ri-He Peng, Ai-Sheng Xiong, Jin-ge Liu, Fang Xu, Cai Bin, Hong Zhu, Quan-Hong Yao —Preceding unsigned comment added by 128.220.159.2 (talk) 20:19, 2 November 2009 (UTC)[reply]

pGEM[edit]

Doesn't the pGEM vector system (Promega) use this technique? I remember I always had to A-tail my insert before ligating it into pGEM. That might be worth mentioning. Pdcook (talk) 16:04, 4 November 2009 (UTC)[reply]

Seems that it does from reading the manual. There are several vector system and kits from different companies that use this technique, the question is should we go into listing all these different kits? --Vishnu2011 (talk) 16:08, 4 November 2009 (UTC)[reply]

We probably shouldn't list all the companies that utilize this technique, but it might be worth mentioning that there are commercially-available vectors that do use TA cloning or some version of it. That might highlight the importance of the technique. Your call. Pdcook (talk) 16:14, 4 November 2009 (UTC)[reply]

Good point. I did mention it a little before "Manufacturers commonly sell TA Cloning "kits" with with a wide range of prepared vectors that have already been linearized and tagged with an overhanging thymine residue.". But I think I will add it in the lead too. Thanks --Vishnu2011 (talk) 16:46, 4 November 2009 (UTC)[reply]

Oh, I missed that line. That actually sounds pretty good as written, but a little expansion might not hurt. The article is looking good! Pdcook (talk) 18:31, 4 November 2009 (UTC)[reply]

Commercializations[edit]

The article had "Commercialized kits with pre-prepared vectors and PCR reagents are currently sold, greatly speeding up the process.". This sounded like an advertizement. I am sure we can say that for ALL tools in molecular biology, there are kits available. It need no special mention on all wikipedia pages, right? Especially not when claims are included like "greatly speeding up the process" when no verifyble link for such a statement is given. 84.112.136.52 (talk) 06:58, 17 October 2013 (UTC)[reply]

Agreed Vishnu2011 (talk) 17:16, 19 October 2013 (UTC)[reply]