Galectin-9

LGALS9
Available structures PDB Human UniProt search: PDBe RCSB List of PDB id codes 2EAK, 2EAL, 2YY1, 2ZHK, 2ZHL, 2ZHM, 2ZHN, 3LSD, 3LSE, 3NV1, 3NV2, 3NV3, 3NV4, 3WLU, 3WV6
Identifiers
Aliases	LGALS9, HUAT, LGALS9A, galectin 9
External IDs	OMIM: 601879 HomoloGene: 32078 GeneCards: LGALS9
Gene location (Human) Chr. Chromosome 17 (human) Band 17q11.2 Start 27,629,798 bp End 27,649,560 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte rectum gallbladder spleen lymph node pylorus appendix right uterine tube upper lobe of left lung right lung n/a More reference expression data BioGPS More reference expression data
Gene ontology Molecular function galactose binding signal transducer activity enzyme binding disaccharide binding carbohydrate binding Cellular component intracellular anatomical structure extracellular region extracellular exosome cytoplasm nucleus extracellular space cytosol Biological process negative regulation of interferon-gamma production response to interleukin-1 toll-like receptor 4 signaling pathway maternal process involved in female pregnancy positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell immune system process positive regulation of CD4-positive, alpha-beta T cell proliferation ERK1 and ERK2 cascade female pregnancy positive regulation of viral entry into host cell positive regulation of monocyte chemotactic protein-1 production natural killer cell tolerance induction negative regulation of chemokine production regulation of interleukin-5 production toll-like receptor 2 signaling pathway positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway negative regulation of mast cell degranulation negative regulation of gene expression positive regulation of interleukin-4 production chemotaxis negative regulation of activated T cell proliferation regulation of interleukin-4 production response to lipopolysaccharide positive regulation of gene expression positive regulation of NF-kappaB transcription factor activity positive regulation of dendritic cell apoptotic process positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma positive regulation of I-kappaB kinase/NF-kappaB signaling p38MAPK cascade negative regulation of natural killer cell mediated cytotoxicity positive regulation of transforming growth factor beta production negative regulation of CD4-positive, alpha-beta T cell proliferation positive regulation of dendritic cell differentiation negative regulation of tumor necrosis factor production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response inflammatory response regulation of p38MAPK cascade mature conventional dendritic cell differentiation positive regulation of dendritic cell chemotaxis positive regulation of activated T cell autonomous cell death Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3965 n/a Ensembl ENSG00000168961 n/a UniProt O00182 n/a RefSeq (mRNA) NM_002308 NM_009587 NM_001330163 n/a RefSeq (protein) NP_001317092 NP_002299 NP_033665 n/a Location (UCSC) Chr 17: 27.63 – 27.65 Mb n/a PubMed search n/a
Wikidata
View/Edit Human

Galectin-9 was first isolated from mouse embryonic kidney in 1997 as a 36 kDa beta-galactoside lectin protein. Human galectin-9 is encoded by the LGALS9 gene.

Function

The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene.

Galectin-9 is one of the most studied ligands for HAVCR2 (TIM-3) and is expressed on various tumor cells. However, it can also interact with other proteins (CLEC7A, CD137, CD40). For example, an interaction with CD40 on T-cells inhibits their proliferation and induces cell death.

Galectin-9 also has important cytoplasmic, intracellular functions and controls AMPK in response to lysosomal damage that can occur upon exposure to endogenous and exogenous membrane damaging agents such as crystalline silica, cholesterol crystals, microbial toxins, proteopathic aggregates such as tau fibrils and amyloids, and signaling pathways inducing lysosomal permeabilization such as those initiated by TRAIL. Mild lysosomal damage, such as that caused by the anti-diabetes drug metformin may contribute to the therapeutic action of metformin by activating AMPK. The mechanism of how Galectin-9 activates AMPK involves recognition of exposed lysosomal lumenal glycoproteins such as LAMP1, LAMP2, SCRAB2, TMEM192, etc., repulsion of deubiquitinating enzyme USP9X, increased K63 ubiquitination of TAK1 (MAP3K7) kinase, which in turn phopshorylates AMPK and activates it. This signaling cascade directly links Galectin-9 intracellular function with ubiqutin systems. Galectin-9, through its regulation of AMPK, a kinase that negatively regulates mTOR, cooperates with Galectin-8-based effects to inactivate mTOR downstream of the lysosomal damaging agents and conditions.

Clinical significance

The expression of galectin-9 has been detected on various hematological malignancies, such as CLL, MDS, Hodgkin and Non-Hodgkin lymphomas, AML or solid tumors, such as lung cancer, breast cancer, and hepatocellular carcinoma.

HAVCR2/ galectin-9 interaction attenuated T-cell expansion and effectors function in tumor microenvironment and chronic infections. Moreover, galectin-9 contributed to tumorigenesis by tumor cell transformation, cell-cycle regulation, angiogenesis, and cell adhesion. The correlative studies analyzing the expression of galectin-9 and malignant clinical features showed controversial results. This can be explained as that galectin-9 can promote tumor immune escape as well as inhibit metastasis by promoting endothelial adhesion. Therefore many factors such as tumor type, stage, and the involvement of different galectins should be take into consideration when correlating the expression level and the malignancy.

Galectin-9, through its cytoplasmic action in control of AMPK, may affect various health conditions impacted by AMPK, including metabolism, obesity, diabetes, cancer, immune responses, and may be a part of the mechanism of action of the widely-prescribed anti-diabetes drug metformin.

Further reading

• Hirashima M, Kashio Y, Nishi N, Yamauchi A, Imaizumi TA, Kageshita T, Saita N, Nakamura T (2004). "Galectin-9 in physiological and pathological conditions". Glycoconjugate Journal. 19 (7–9): 593–600. doi:10.1023/B:GLYC.0000014090.63206.2f. PMID 14758084. S2CID 11006101.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Matsumoto R, Matsumoto H, Seki M, Hata M, Asano Y, Kanegasaki S, Stevens RL, Hirashima M (July 1998). "Human ecalectin, a variant of human galectin-9, is a novel eosinophil chemoattractant produced by T lymphocytes". The Journal of Biological Chemistry. 273 (27): 16976–84. doi:10.1074/jbc.273.27.16976. PMID 9642261.
• Matsumoto R, Hirashima M, Kita H, Gleich GJ (February 2002). "Biological activities of ecalectin: a novel eosinophil-activating factor". Journal of Immunology. 168 (4): 1961–7. doi:10.4049/jimmunol.168.4.1961. PMID 11823532.
• Kageshita T, Kashio Y, Yamauchi A, Seki M, Abedin MJ, Nishi N, Shoji H, Nakamura T, Ono T, Hirashima M (June 2002). "Possible role of galectin-9 in cell aggregation and apoptosis of human melanoma cell lines and its clinical significance". International Journal of Cancer. 99 (6): 809–16. doi:10.1002/ijc.10436. PMID 12115481. S2CID 205934076.
• Imaizumi T, Kumagai M, Sasaki N, Kurotaki H, Mori F, Seki M, Nishi N, Fujimoto K, Tanji K, Shibata T, Tamo W, Matsumiya T, Yoshida H, Cui XF, Takanashi S, Hanada K, Okumura K, Yagihashi S, Wakabayashi K, Nakamura T, Hirashima M, Satoh K (September 2002). "Interferon-gamma stimulates the expression of galectin-9 in cultured human endothelial cells". Journal of Leukocyte Biology. 72 (3): 486–91. doi:10.1189/jlb.72.3.486. PMID 12223516. S2CID 17010289.
• Asakura H, Kashio Y, Nakamura K, Seki M, Dai S, Shirato Y, Abedin MJ, Yoshida N, Nishi N, Imaizumi T, Saita N, Toyama Y, Takashima H, Nakamura T, Ohkawa M, Hirashima M (November 2002). "Selective eosinophil adhesion to fibroblast via IFN-gamma-induced galectin-9". Journal of Immunology. 169 (10): 5912–8. doi:10.4049/jimmunol.169.10.5912. PMID 12421975.
• Kashio Y, Nakamura K, Abedin MJ, Seki M, Nishi N, Yoshida N, Nakamura T, Hirashima M (April 2003). "Galectin-9 induces apoptosis through the calcium-calpain-caspase-1 pathway". Journal of Immunology. 170 (7): 3631–6. doi:10.4049/jimmunol.170.7.3631. PMID 12646627.
• Abedin MJ, Kashio Y, Seki M, Nakamura K, Hirashima M (May 2003). "Potential roles of galectins in myeloid differentiation into three different lineages". Journal of Leukocyte Biology. 73 (5): 650–6. doi:10.1189/jlb.0402163. PMID 12714580. S2CID 21337581.
• Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S (May 2003). "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways". Oncogene. 22 (21): 3307–18. doi:10.1038/sj.onc.1206406. PMID 12761501. S2CID 38880905.
• Irie A, Yamauchi A, Kontani K, Kihara M, Liu D, Shirato Y, Seki M, Nishi N, Nakamura T, Yokomise H, Hirashima M (April 2005). "Galectin-9 as a prognostic factor with antimetastatic potential in breast cancer". Clinical Cancer Research. 11 (8): 2962–8. doi:10.1158/1078-0432.CCR-04-0861. PMID 15837748. S2CID 15041189.
• Kasamatsu A, Uzawa K, Nakashima D, Koike H, Shiiba M, Bukawa H, Yokoe H, Tanzawa H (August 2005). "Galectin-9 as a regulator of cellular adhesion in human oral squamous cell carcinoma cell lines". International Journal of Molecular Medicine. 16 (2): 269–73. doi:10.3892/ijmm.16.2.269. PMID 16012760.
• Dai SY, Nakagawa R, Itoh A, Murakami H, Kashio Y, Abe H, Katoh S, Kontani K, Kihara M, Zhang SL, Hata T, Nakamura T, Yamauchi A, Hirashima M (September 2005). "Galectin-9 induces maturation of human monocyte-derived dendritic cells". Journal of Immunology. 175 (5): 2974–81. doi:10.4049/jimmunol.175.5.2974. PMID 16116184.
• Zhu C, Anderson AC, Schubart A, Xiong H, Imitola J, Khoury SJ, Zheng XX, Strom TB, Kuchroo VK (December 2005). "The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity". Nature Immunology. 6 (12): 1245–52. doi:10.1038/ni1271. PMID 16286920. S2CID 24886582.
• van de Weyer PS, Muehlfeit M, Klose C, Bonventre JV, Walz G, Kuehn EW (December 2006). "A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9". Biochemical and Biophysical Research Communications. 351 (2): 571–6. doi:10.1016/j.bbrc.2006.10.079. PMID 17069754.

External links

• Overview of all the structural information available in the PDB for UniProt: O00182 (Galectin-9) at the PDBe-KB.