File:Comparison of dimension-unlimited epifluorescence imaging cycler microscopy (ICM) and standard three-parameter fluorescence microscopy..jpg
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Summary
Comparison of dimension-unlimited fluorescence imaging cycler microscopy (ICM) and standard three-parameter fluorescence microscopy
| Description |
English: (a) Dermoepithelial junction (human skin tissue section) measured by three-parameter fluorescence microscopy. (b) ICM, same location as in (a) resolves the basal lamina densa (LD) as a giant stoichiometrically controlled supermolecule and discerns the three basal lamina layers (lamina lucida, LL; lamina densa, LD, and lamina fibroreticularis, LF) as indicated by comparison with a transmission electron micrograph (c). (d) Profiles of 100 distinct biomolecules co-mapped by ICM. (e) Three-dimensional network of supermolecules (different colours, measured by ICM) on the cell surface of a single human blood T-lymphocyte[1][2]. (f) More than 7,000 different protein clusters in a human liver cell measured by ICM[3]. Scale bars: 10 µm (a,b); 50 nm (c); 1 µm (e); 10µm (f). |
| Date | |
| Source | Own work |
| Author | XXX-MPRR-XXX |
References
- ↑ Friedenberger, Manuela; Bode, Marcus; Krusche, Andreas; Schubert, Walter (September 2007). "Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures". Nature Protocols 2 (9): 2285–2294. DOI:10.1038/nprot.2007.320.
- ↑ Schubert, W (2014). "Systematic, spatial imaging of large multimolecular assemblies and the emerging principles of supramolecular order in biological systems.". Journal of Molecular Recognition 27 (1): 3-18. DOI:10.1002/jmr.2326.
- ↑ Schubert, Walter; Bonnekoh, Bernd; Pommer, Ansgar J; Philipsen, Lars; Böckelmann, Raik; Malykh, Yanina; Gollnick, Harald; Friedenberger, Manuela; Bode, Marcus; Dress, Andreas W M (1 October 2006). "Analyzing proteome topology and function by automated multidimensional fluorescence microscopy". Nature Biotechnology 24 (10): 1270–1278. DOI:10.1038/nbt1250.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 14:09, 5 February 2014 | | 1,200 × 3,959 (1.68 MB) | XXX-MPRR-XXX | sub figure (a) - (f) description added |
| 11:39, 5 February 2014 |  | 1,200 × 3,959 (1.62 MB) | XXX-MPRR-XXX | wrong numbering of sub figure a) and b) (items were switched) | |
| 14:35, 4 February 2014 |  | 1,200 × 3,978 (1.63 MB) | XXX-MPRR-XXX | User created page with UploadWizard |
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